Past Funding

 

NIH UG3TR002158 Himmelfarb (PI) Shankland (Co-I) (07/25/17-06/30/19)

A Microphysiological System for Kidney Disease Modeling and Drug Efficacy Testing

The goal of this application is to model important human kidney diseases and promote identification of safe and effective treatments. To

achieve this goal, we have established a multidisciplinary investigative team with expertise in kidney physiology and pathology, cellular

and molecular biology, systems pharmacology and toxicology, biomarker discovery and evaluation, biomedical engineering, microfluidics,

matrix biology, genomics, computational biology, and biostatistics.

NIDDK (04/01/2018-03/31/2019)

12th International Podocyte Conference

Conference, with the goal to ultimately reduce the burden of disease in this patient population through improved disease

detection and treatment.

Department of Defense   Shankland, Punn (MPI)  (06/15/2016-12/14/2018)

Development of cell therapies for FSGS

The goal of this project is to develop a clinically plausible podocyte  regeneration therapy using nanoparticle-enhanced autologous uRPCs

(NP-uRPCs).

NIH R01 5 R01 DK093493-02 Shankland (PI)   (9/03/2012-6/30/2017)                                                                                    

Pericyte-endothelial cross talk in vascular stability after kidney injury

These studies will investigate the mechanisms by which pericytes nurture kidney blood vessels and the mechanisms by which they detach

in response to injury and thereafter fail to nurture. In understanding  these processes we hope to develop new therapies to treat kidney

diseases.

   

NIH/NIDDK  5 R01 DK083391-01A2 Alpers (PI) Shankland (Co-I)   (9/15/2011-8/31/2016)      

Podocyte depletion and regeneration during evolution and reversal of diabetic nephropathy

This grant explores mechanisms underlying reversibility (replacement of kidney cell populations that are typically lost in human and

experimental diabetic kidney disease) with emphasis on the podocyte, a unique cell type in the kidney. This grant also focuses on testing

new therapies, based on correcting metabolic injuries to mitochondria (an organelle present within all cells), that are specifically directed

to podocyte mitochondria, as a way to promote reversal of diabetic kidney disease

NIH/NIDDK   5 R01 DK056799 Shankland  (PI) (9/1/2011-4/30/2016)

Cell Cycle and Podocytes    

The goal of the first aim of this competitive renewal is to show a new paradigm for cell cycle protein function, based on our preliminary

data In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein research. The

overall goal of the second aim is to show new regulatory paradigms for cyclin I-p35-cdk5, which play a fundamental role in glomerular and

brain development.   In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein

research.

NIH – R01- DK083391 Shankland (Co-I) (9/15/2011-8/31/2015)

Podocyte depletion and regeneration during evolution and reversal of diabetic nephropathy

This grant explores mechanisms underlying reversibility (replacement of kidney cell populations that are typically lost in human and

experimental diabetic kidney disease) with emphasis on the podocyte, a unique cell type in the kidney. This grant also focuses on testing

new therapies, based on correcting metabolic injuries to mitochondria (an organelle present within all cells), that are specifically directed

to podocyte mitochondria, as a way to promote reversal of diabetic kidney disease.

NIH Basic Training Grant T32 DL07467-21 Shankland (PI) (09/01/2009–8/31/13)

Nephrology Training 03

This is a training grant that supports research training for three M.D. post-graduate fellows who undergo periods of both renal and basic

science research training.

NIH R24 1R24DK094768 Shankland (PI) (8/01/2012 – 7/31/2014)

Kidney Progenitor Cells in Disease

These initial studies will study progenitor cells in the adult kidney that have the capacity to generate new kidney cells particularly of the

blood cells of the kidney. Investigators will determine how important these progenitors are in regeneration after kidney damage.

Biogen, IDEC MA Inc. Shankland (PI) (4/1/2013-6/31/2014)

Fn14 biology for discovery of new targets that regulate the fate of pericytes

NIH R01 DK-084077 Shankland (PI) (9/1/2009 – 5/31/2014)

The role of macrophage delivered WNT signaling in kidney injury and repair

Determine the role of macrophage delivered Wnt signaling to epithelial cells in kidney repair after ischemic injury, and the role of Wnt

signaling from macrophages to fibroblasts in fibrosis progression.

Boehringer Ingelheim Pharmaceuticals Inc. Shankland (PI) (5/02/2013-05/01/2014)

The Role of CTGF Mouse and Human Pericyte Activation and Disease

American Heart Association 11GRNT7810021 Shankland (PI) (7/1/2011-6/30/13)

Cell Cycle Regulation of Podocyte Proteins

The overall goal of this grant proposal is to prove that both slit diaphragm and actin-binding proteins are Regulated by the cdk5-cyclin I-

p35 complex, and that any alterations in this complex such as occurs in disease, leads to proteinuria and glomerulosclerosis.

NIH/NIDDK 1 R13 DK095622 Shankland (PI) (01/01/2012-12/31/2012)

9th International Podocyte Conference

This R13 grant application is written to support the the 9th International Podocyte Conference, the major goal of which is to substantially

advance the clinical and research aspects of podocyte diseases

NIH-RO1- Renewal Shankland (PI) (7/1/2011 - 6/30/2016)

Cell Cycle and Podocyte Apoptosis

Studies are designed to determine the role of Cyclin I and CDK5, and when active, this complex enhances Survival of terminally differential

cells such as podocytes. The signaling pathways will be delineated. Cell culture and experimental models will be used to delineate this

further.

               

NIH-RO1 Shankland (PI) (7/1/10 - 6/30/15)

Podocyte Progenitor Cells

The focus of this grant application is to delineate how terminally differentiated podocyte cell number is Restored following injury in

glomerular disease. Based on our recent data and preliminary data, we have compelling evidence that renin-expressing juxtaglomerular

cells and parietal epithelial cells both serve as local progenitor cells. We will show that they both switch their phenotype in disease, and

ultimately become podocytes.
 

NIH/NIDDK 2R01DK056799 Shankland (PI) (09/01/2011-04/30/2012)

Cell Cycle and Podocytes

The goal of the first aim of this competitive renewal is to show a new paradigm for cell cycle protein function, based on our preliminary

data In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein research. The

overall goal of the second aim is to show new regulatory paradigms for cyclin I-p35-cdk5, which play a fundamental role in glomerular and

brain development. In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein

research.
 

NIH ARRA Grant  (DK056799-07S1)  Shankland (PI)  (09/21/09-08/31/11)

Cell Cycle and Podocyte Apoptosis

Studies are designed to determine the role of Cyclin I and CDK5, and when active, this complex enhances survival of terminally differential

cells such as podocytes. The signaling pathways will be delineated. Cell culture and experimental models will be used to delineate this

further.
 

NIH R01 Grant (DK056799-05A1) Shankland (PI) (09/30/07 – 6/30/11)

Cell Cycle and Podocyte Apoptosis

The overall goal of this grant is to delineate new paradigms in the regulation of podocyte survival and death, so that ultimately new

strategies can be developed to prevent podocyte loss, enhance kidney survival, and reduce kidney disease.
 

 

NIH NIDDK ARRA R21 (DK081835-01A2) Shankland (PI) (08/15/09 – 06/30/11)

New Thoughts on Parietal Cells

The precise biological role of parietal epithelial cells (PECs) are not well understood in health or disease. The main goal of this grant is to

show that PECs are required to limit filtered proteins ‘escaping’ into the peri-glomerular space. We will test the hypotheses that the intra-

cellular tight junctions in PECs, together with the underlying Bowman’s Basement membrane, forms a second barrier to proteinuria. We will

also test the hypothesis that PECs actively take up filtered albuminuria which injures them by inducing apoptosis.
 

1 F32 DK072788-01 Shankland (PI) (9/19/05-12/30/07)

HHS Logar Fellow


 DHHS 5901 Letter of Credit (LOC) Shankland (PI) (4/1/2008-3/31/09)

7th Intl Podocyte Conference

This grant will be used to support the travel of junior investigators to attend scientific conference.
 

American Heart Association – Established Investigator Award 0340129N Shankland (PI) (1/01/03– 12/31/08)

Role of Cell Cycle in Podocyte Injury

The proposed studies are designed to test novel aspects of cell cycle proteins in disease, so that potential therapeutic interventions can be

designed to reduce the incidence of renal failure.

NIH R01 Grant 51096-05 A2 Shankland (PI) (09/01/04-06/30/08)

Cell Cycle Control in Glomerular Disease

This competitive renewal will continue to examine the role of specific cell cycle regulatory proteins in podocyte proliferation and

differentiation.

5 F32DK070434-02 Shankland (PI) (9/12/05-8/30/08)

Immune Renal Injury IA

Tests the central hypothesis that a novel mechanism limiting podocyte proliferation is DNA damage.

NIH/NIDDK R13 Shankland (PI)

6th International Podocyte Conference, Helsinki, Finland

This grant will be used to support the travel of junior investigators to attend this scientific meeting.

American Diabetes Association (7-04-RA-107-80-0572) Shankland (PI) (07/01/04-06/30/06)

Role of Podocytes in Diabetic Nephropathy

The overall goal of this grant is to delineate the mechanisms underlying the abnormalities in podocytes, utilizing cultured cells and animal

models of diabetic nephropathy.

NIH R01 grant DK 60525-02 Shankland (PI) (07/01/02-06/30/06)

Mechanisms of Podocyte Injury

The major goals of this grant are to study the effect of stress-tension-induced injury on podocytes, and also to determine the role of novel

cell cycle regulatory proteins in podocyte disease.

AMGEN – Applied Molecular Genetics 200516848 Shankland (PI) (11/28/05-12/31/07)

Proteinuria Darbepoetin

The goals of this grant are to test the hypotheses that darbepoetin prevents the decrease in nephrin and podocin levels and thereby limits

proteinuria.

AMGEN - Applied Molecular Genetics 200420479 Shankland (PI) (09/27/04-09/26/06)

Role of Erythropeitin (EPO) in Proteinuria

The overall goal of this grant is to test whether a decrease in endogenous EPO in renal disease augments loss of podocytes differentiation,

preventing progressive proteinuria and scarring.

 

NIH R01 grant DK 56799-04 Shankland (PI) (05/01/00-02/28/05)

Cell Cycle Proteins and Glomerular Apoptosis

The major goal of this project is to show novel roles for specific cell cycle proteins in glomerular cell apoptosis beyond that of proliferation.
 

NIH/NIDDK R13 DK069139-01 Shankland (PI) (06/01/04-06/30/05)

“5th International Podocyte Conference”

This grant was used to support the travel of junior investigators to attend this scientific meeting.

Juvenile Diabetes Research Foundation (JDRF)  1-2001-395 Shankland (PI) (01/01/01-12/31/03)

The Role of Cyclin Kinase Inhibitors in Diabetic Glomerular Hypertrophy

The major goal of this project will be to determine the nuclear mechanisms underlying the development of diabetic hypertrophy, with a

focus on specific CDK inhibitors.

NIH R01 grant DK 47659-10 Shankland (PI) (09/01/98-08/31/03)

Immunological Renal Diseases: Subproject #2, “The Role of Cell Cycle Proteins in Glomerular Epithelial Cell Growth” 
 

The major goal of this project is to test the hypothesis that specific CDK-inhibitors p57-WT1 determine the proliferative capacity of the

glomerular visceral epithelial cell.

NIH R01 grant DK 51096 Shankland (PI) (06/01/96-05/31/04)

Cell Cycle Control in Glomerular Disease

The major goal of this grant is to determine the expression and activity of specific cell cycle proteins in mesangial cell proliferation in vitro

and in vivo.
 

NIH Center Grant P50-DK 47659 Shankland (PI) (09/01/98-08/31/03)

George M. O’Brien Kidney Research Center

Subproject #2: The Role of Cell Cycle Proteins in Glomerular Epithelial Cell Growth

The major goal of this project is to test the hypothesis that WT1 determines the proliferative capacity of the glomerular visceral epithelial

cell.

NIH (R01) Grant; DK 52121 Shankland (PI) (01/01/97-12/31/01)

Role of the Glomerular Endothelial Cell in Hemolytic Uremic Syndrome (HUS)

The goal was to determine the mechanisms of endothelial cell proliferation and apoptosis.
 

C. V. Therapeutics, Inc. Grant Shankland (PI) (01/01/00-12/31/00)

Role of Cell Cycle Proteins in Hemodialysis Vascular Stenosis

The study was designed to determine the role of cell cycle proteins in vascular access stenosis.