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What we've been up to

in the

Shankland Lab!

 

 


Watch our video and learn about the unique and rigorous
Nephrology Fellowship Program at the University of Washington


Grants

 

NIH/NIA  (Shankland) 08/15/2016-03/31/2021

Reduced Glomerular Progenitors Impair Regeneration in Aged Kidney

The grant will define how the decline in podocyte number with advancing age cannot adequately be replaced by their neighboring parietal

epithelial cell progenitors, which leads to kidney scarring.

 

DOD  (Shankland) PI 09/30/2016-09/29/2019         

New podocyte-targeted treatments for focal segmental glomerulosclerosis (FSGS)


Our overall goals are to improve drug biodistribution directly to podocytes while decreasing systemic exposure to active drug, and to demonstrate

drug efficacy and safety in an animal model of FSGS. To meet these objectives we propose the following aims: Aim 1. Optimize and characterize cyclic

“sunflower” polymer kidney accumulation in a mouse model of FSGS. The goal of this aim is to synthesize and optimize a drug delivery vehicle that

preferentially distributes to and is retained in the kidney after injection.

 

DOD  PR151965   (Shankland and Pun)   6/15/2016-12/14/2018

Development of Cell Therapies for FSGS

The goal of this project is to develop a clinically plausible podocyte regeneration therapy using nanoparticle-enhanced autologous

uRPCs (NP-uRPCs).

 

NIH/NIDDK  1 UH2 DK107343-01   (Shankland and Zheng)   07/01/2015-06/30/2020  

Rebuilding the glomerular filtration barrier by regenerating adult podocytes

 (Re)BuildingAKidney.org

Podocytes are cells in the kidney’s glomerular filtering units that limit the passage of proteins from the blood in to the urine. As adults, they

cannot proliferate to replace , and therefore they are reliant on other stem cells for their regeneration. In this grant, we will study such stem

cells in podocyte repair to rebuild a kidney.

 

NIH/NIDDK 1 R01 DK097598-01A1 (Shankland) PI   08/05/2014-04/30/2019                                                                            

Juxta-glomerular cells serve as glomerular epithelial cell progenitors in glomerular disease

The purpose of this proposal is to study the existing problem of age-related podocyte depletion in a completely new context. The goal is to

prove that with advancing age, kidney regeneration, and thus repair, is inadequate because progenitors are unable to replace and restore

glomerular podocytes. We anticipate that the results will provide compelling evidence for a new paradigm in aging kidneys in which recently

identified progenitors are unable to adequately regenerate to replace podocytes, which leads to glomerulosclerosis and reduced kidney

function.

 

NIH/NIDDK   5 T32 DL07467-31 (Shankland) PI  7/15/1993-6/30/2019

 Training in Renal Disease

This is an institutional grant to train fellows and postdocs in kidney research.

 

1UG3 TR002158     (Himmelfarb) PI   (Shankland) Co-I   07/25/17-06/30/19                       

A Microphysiological System for Kidney Disease Modeling and Drug Efficacy Testing

The goal of this application is to model important human kidney diseases and promote identification of safe and effective treatments.

To achieve this goal, we have established a multidisciplinary investigative team with expertise in kidney physiology and pathology, cellular and

molecular biology, systems pharmacology and toxicology, biomarker discovery and evaluation, biomedical engineering, microfluidics, matrix

biology, genomics, computational biology, and biostatistics. If successful, ultimately in vitro models that recapitulate critical aspects of kidney

physiological function, response to injury, and repair could contribute greatly to drug discovery and development, and could ultimately enable

‘virtual clinical trials’ for candidate therapeutics.


 NIH/NIDDK U2CDK114886 (Himmelfarb, Iyengar, Kretzler) MPI (Shankland) Co-I 09/15/17-06/30/22

Central Hub for Kidney Precision Medicine

The overarching objective of the KPMP Central Hub is to facilitate logistics and operations required to promote scientific rigor, patient safety,

and the successful interdisciplinary team science necessary to for major advances in kidney precision medicine.


Shankland-at-podium.jpg

12th International Podocyte Conference


 Highlight on Papers 

Characterization of Glomerular Sox9+ Cells in Anti-Glomerular Basement Membrane Nephritis in the Rat

PMID: 30201496

Prochnicki A, Amann K, Wegner M, Sock E, Pfister E, Shankland SJ, Pippin JW, Daniel C

Cells of NG2 lineage increase in glomeruli of mice following podocyte depletion

PMID: 30019931

Suzuki T, Eng DG, McClelland AD, Pippin JW, Shankland SJ

Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy

PMID: 29991751

 Chozinski, T, Mao, C, Halpern, A, Pippin, J, Alpers, C, Shankland, SJ, Najafian, B, Vaughan

Krüppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation

PMID: 29925693

Estrada, CC, Paladugu, P, Guo, Y, Pace, J, Revelo, MP, Salant, DJ, Shankland, SJ, D’Agati, VD, Mehrotra, A,

Cardona, S, Bialkowska, AB ,Yang, VW,   He, JC, Mallipattu, SK.

Glomerular disease augments kidney accumulation of synthetic anionic polymers

PMID: 29891232

Liu GW, Prossnitz AN, Eng DG, Cheng Y, Subrahmanyam N, Pippin JW, Lamm RJ, Ngambenjawong C, Ghandehari H, Shankland SJ, Pun SH

High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping

PMID: 29779890

Czerniecki SM, Cruz NM, Harder JL, Menon R, Annis J, Otto EA, Gulieva RE, Islas LV, Kim YK, Tran LM, Martins TJ,

Pippin JW, Fu H, Kretzler M, Shankland SJ, Himmelfarb J, Moon RT, Paragas N, Freedman BS

Sex differences in transcriptomic profiles in aged kidney cells of renin lineage

PMID: 29676999 

Wang Y, Eng DG, Pippin JW, Gharib SA, McClelland A, Gross KW, Shankland SJ

 

More about our Research 


ASN - Kidney Week - New Orleans


G.Liu_crop2.jpg

Congratulations to Gary
on passing his general examination

Gary is a PhD candidate!



Andrea Largent discussing her research

20th Annual Undergraduate Research Symposium


Shankland Lab Research featured on cover of Kidney International

  Shankland Lab Research featured on cover of  Kidney International

Dr. Mariya Sweetwyne's paper accepted to Kidney International

Mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age

PMID: 28063595


New Funding for FSGS Research

FY 2015 Investigator-Initiated Research Award – Partnering PI Option

Suzie Pun – University of Washington

Stuart Shankland – University of Washington


Select Presentations

Society of Toxicologic Pathology Annual Meeting - 2018

International Podocyte Conference - 2018

American Society of Nephrology Annual Meeting, New Orleans, LA - 2017

“Podocyte Regeneration from Renin Lineage Cells”

American Society of Nephrology Annual Meeting, San Diego, CA - 2015

"What is Aging Nephropathy?" 

"Role of Parietal Cells Following Glomerular Injury"

New York Renal Society - 2015

German Society of Nephrology - 2014

International Podocyte Conference - 2014

National Kidney Foundation Annual Meeting - 2014

SSCI Annual Meeting - 2014



Posters

Kaverina, Natalya

Multi-Clonal Population of Cells of Renin Lineage (CoRL) Transdifferentiate into Podocytes and PECs in Experimental FSGS

 

Lichtnekert, Julia

RAS Inhibition Enhances Proliferation and Migration of Cells of Renin Lineage (CoRL) as Progenitors in Experimental FSGS



The Lab Gang


Shankland Lab