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Nephrology Fellowship Program at the University of Washington
NIH/NIA (Shankland) 08/15/2016-03/31/2021
Reduced Glomerular Progenitors Impair Regeneration in Aged Kidney
The grant will define how the decline in podocyte number with advancing age cannot adequately be replaced by their neighboring parietal
epithelial cell progenitors, which leads to kidney scarring.
DOD (Shankland) PI 09/30/2016-09/29/2019
New podocyte-targeted treatments for focal segmental glomerulosclerosis (FSGS)
Our overall goals are to improve drug biodistribution directly to podocytes while decreasing systemic exposure to active drug, and to demonstrate
drug efficacy and safety in an animal model of FSGS. To meet these objectives we propose the following aims: Aim 1. Optimize and characterize cyclic
“sunflower” polymer kidney accumulation in a mouse model of FSGS. The goal of this aim is to synthesize and optimize a drug delivery vehicle that
preferentially distributes to and is retained in the kidney after injection.
DOD PR151965 (Shankland and Pun) 6/15/2016-12/14/2018
Development of Cell Therapies for FSGS
The goal of this project is to develop a clinically plausible podocyte regeneration therapy using nanoparticle-enhanced autologous
NIH/NIDDK 1 UH2 DK107343-01 (Shankland and Zheng) 07/01/2015-06/30/2020
Rebuilding the glomerular filtration barrier by regenerating adult podocytes
Podocytes are cells in the kidney’s glomerular filtering units that limit the passage of proteins from the blood in to the urine. As adults, they
cannot proliferate to replace , and therefore they are reliant on other stem cells for their regeneration. In this grant, we will study such stem
cells in podocyte repair to rebuild a kidney.
NIH/NIDDK 1 R01 DK097598-01A1 (Shankland) PI 08/05/2014-04/30/2019
Juxta-glomerular cells serve as glomerular epithelial cell progenitors in glomerular disease
The purpose of this proposal is to study the existing problem of age-related podocyte depletion in a completely new context. The goal is to
prove that with advancing age, kidney regeneration, and thus repair, is inadequate because progenitors are unable to replace and restore
glomerular podocytes. We anticipate that the results will provide compelling evidence for a new paradigm in aging kidneys in which recently
identified progenitors are unable to adequately regenerate to replace podocytes, which leads to glomerulosclerosis and reduced kidney
NIH/NIDDK 5 T32 DL07467-31 (Shankland) PI 7/15/1993-6/30/2019
Training in Renal Disease
This is an institutional grant to train fellows and postdocs in kidney research.
1UG3 TR002158 (Himmelfarb) PI (Shankland) Co-I 07/25/17-06/30/19
A Microphysiological System for Kidney Disease Modeling and Drug Efficacy Testing
The goal of this application is to model important human kidney diseases and promote identification of safe and effective treatments.
To achieve this goal, we have established a multidisciplinary investigative team with expertise in kidney physiology and pathology, cellular and
molecular biology, systems pharmacology and toxicology, biomarker discovery and evaluation, biomedical engineering, microfluidics, matrix
biology, genomics, computational biology, and biostatistics. If successful, ultimately in vitro models that recapitulate critical aspects of kidney
physiological function, response to injury, and repair could contribute greatly to drug discovery and development, and could ultimately enable
‘virtual clinical trials’ for candidate therapeutics.
NIH/NIDDK U2CDK114886 (Himmelfarb, Iyengar, Kretzler) MPI (Shankland) Co-I 09/15/17-06/30/22
Central Hub for Kidney Precision Medicine
The overarching objective of the KPMP Central Hub is to facilitate logistics and operations required to promote scientific rigor, patient safety,
and the successful interdisciplinary team science necessary to for major advances in kidney precision medicine.
12th International Podocyte Conference
Highlight on Papers
Characterization of Glomerular Sox9+ Cells in Anti-Glomerular Basement Membrane Nephritis in the Rat
Prochnicki A, Amann K, Wegner M, Sock E, Pfister E, Shankland SJ, Pippin JW, Daniel C
Cells of NG2 lineage increase in glomeruli of mice following podocyte depletion
Suzuki T, Eng DG, McClelland AD, Pippin JW, Shankland SJ
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy
Chozinski, T, Mao, C, Halpern, A, Pippin, J, Alpers, C, Shankland, SJ, Najafian, B, Vaughan
Krüppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation
Estrada, CC, Paladugu, P, Guo, Y, Pace, J, Revelo, MP, Salant, DJ, Shankland, SJ, D’Agati, VD, Mehrotra, A,
Cardona, S, Bialkowska, AB ,Yang, VW, He, JC, Mallipattu, SK.
Glomerular disease augments kidney accumulation of synthetic anionic polymers
Liu GW, Prossnitz AN, Eng DG, Cheng Y, Subrahmanyam N, Pippin JW, Lamm RJ, Ngambenjawong C, Ghandehari H, Shankland SJ, Pun SH
High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping
Czerniecki SM, Cruz NM, Harder JL, Menon R, Annis J, Otto EA, Gulieva RE, Islas LV, Kim YK, Tran LM, Martins TJ,
Pippin JW, Fu H, Kretzler M, Shankland SJ, Himmelfarb J, Moon RT, Paragas N, Freedman BS
Sex differences in transcriptomic profiles in aged kidney cells of renin lineage
Wang Y, Eng DG, Pippin JW, Gharib SA, McClelland A, Gross KW, Shankland SJ
More about our Research
ASN - Kidney Week - New Orleans
Gary earned his PhD
Andrea Largent discussing her research
20th Annual Undergraduate Research Symposium
Shankland Lab Research featured on cover of Kidney International
Dr. Mariya Sweetwyne's paper accepted to Kidney International
Mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age
Society of Toxicologic Pathology Annual Meeting - 2018
International Podocyte Conference - 2018
American Society of Nephrology Annual Meeting, New Orleans, LA - 2017
“Podocyte Regeneration from Renin Lineage Cells”
American Society of Nephrology Annual Meeting, San Diego, CA - 2015
"What is Aging Nephropathy?"
"Role of Parietal Cells Following Glomerular Injury"
New York Renal Society - 2015
German Society of Nephrology - 2014
International Podocyte Conference - 2014
National Kidney Foundation Annual Meeting - 2014
SSCI Annual Meeting - 2014
Multi-Clonal Population of Cells of Renin Lineage (CoRL) Transdifferentiate into Podocytes and PECs in Experimental FSGS
RAS Inhibition Enhances Proliferation and Migration of Cells of Renin Lineage (CoRL) as Progenitors in Experimental FSGS